Search 2004, 80 (1): 41-46. As a result physicians usually prescribe broad spectrum antibiotics which are unnecessary and very expensive for patients. Scope for advancement in applications and chemometric approach. This justifies the need for studies which can enable to make distinction between microorganism and human cells. Cytometry. 10.1023/B:JOFL.0000008059.74052.3c.Tourkya : Fluorescence Spectroscopy as a Promising Tool for a Polyphasic Approach to Pseudomonad Taxonomy. But, there is need for further studies and clinical trials to validate this new diagnostic technique.At present, Fluorescence spectroscopy is being applied in medical microbiology field for various purposes. It will help to design invasive and non invasive techniques for microorganism's diagnosis inside the body cavities by use of fiber optic devices.At present, nearly all the diagnostic techniques and methods used for microorganism's diagnosis are not perfect and have some limitations. News-Medical. You can also search for this author in In addition to his interest in clinical, research and lecturing activities, his immense passion for medical writing and scientific communication goes back to his student days. He enjoys contributing back to the community. "Spectroscopy Applications". 2005, 9 (6): 633-8.Sorrell Matthew: Bacteria identification of otitis media with fluorescence spectroscopy. 2006, 1: 121-126.

10.1002/(SICI)1097-0320(19990701)36:3<247::AID-CYTO15>3.0.CO;2-#.Klinger J, Friedrich T: Site-specific interaction of thrombin and inhibitors observed by fluorescence correlation spectroscopy. Its potential applications range from archaeology to modern nanotechnology. News-Medical.Net provides this medical information service in accordance There are many studies which indicate that Fluorescence spectroscopy is promising diagnostic technique with high sensitivity and specificity for microorganisms associated diseases diagnosis with the help of spectroscopic fingerprints. 1994, 52: 31-8. Bacteria are cultured for at least one day and then diagnosis is made.

Saunders Co., 808-3Köhler : Attachment of VLDL Receptors to an Icosahedral Virus along the 5-fold Symmetry Axis: Multiple Binding Modes Evidenced by Fluorescence Correlation Spectroscopy. 2009, 58 (8): 39-46. But, there are many questions which remain to be answered like if biological sample contains more than one microorganism, then how it will affect the spectral signature appearance and how to interpret these spectral for making definite diagnosis. Most of these techniques are laborious, time consuming and with chances of false positive or false negative results. In many research studies, fluorescence emission spectra derived from autofluorescence property of many medically important bacteria make it possible to distinguish between various bacterial species and also enable to classify the bacteria into genus, species and groups. 1999, Kluwer Academic/Plenum Publishers, New York, 2Bhatta H, Goldys EM, Learmonth RP: Use of fluorescence spectroscopy to differentiate yeast and bacterial cells. The combination of fluorescence spectroscopy and chemometric analysis has been demonstrated to have significant potential to measure physical and chemical properties of dairy products.

Also, future studies should be directed to determine the specific spectral regions which will be suitable for identification of specific microorganisms. It warrants the need of a diagnostic technique which can overcome these limitations and problems. Further research is required to establish flexible and portable spectroscopic devices which can be integrated in daily medical practice.There is need for reference libraries for spectral signatures of individual microorganism. Diagnosis of fungal infection is made either by morphological examination of fungi or by biochemical and molecular biology techniques [Studies indicate that Fluorescence spectroscopy may be a novel diagnostic tool to detect viruses. Studies indicate high sensitivity and specificity rate which makes Fluorescence spectroscopy an ideal diagnostic tool for medical microbiology field.

Infectious diseases are caused by microorganisms such as bacteria, viruses, fungi and parasites. 10.1007/s00253-005-0309-y.Leblanc Ludovic, Dufour Eric: Monitoring the identity of bacteria using their intrinsic fluorescence. By continuing to browse this site you agree to our use of cookies. with these We use cookies to enhance your experience. You can also search for this author in Gynecol Oncol.

Waidhofen/Thaya", Vienna.

Also, microorganisms like bacteria have many chemicals which are same like in human cells and in extracellular space, thus body fluids samples may contain same chemicals as found in microorganisms. There are many diagnostic methods and techniques available for microorganisms associated diseases. The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical. 2005, 1 (4): 879-883.Ramanujam N, Mitchell MF, Mahadevan A, Thomsen S, Silva E, Richards-Kortum R: luorescence spectroscopy: A diagnostic tool for cervical intraepithelial neoplasia. 2003, 13 (5): 489-493.

This will be very helpful for comparison with spectral signatures from an unknown microorganism sample. Studies indicate that Fluorescence spectroscopy have great potential to become an excellent and perfect diagnostic technique for microorganisms. Spectroscopy Applications.

2005, 2: 155-163.Giana HE: Rapid Identification of Bacterial Species by Fluorescence Spectroscopy and Classification Through Principal Components Analysis. You can also search for this author in By this modification, infections in many body parts can be detected with ease. 10.1007/s00284-008-9263-0.Rippon JW: Medical Mycology: The Pathogenic Fungi and the Pathogenic Actinomycetes. Also, fiber optic systems may be integrated with this spectroscopic technique to diagnose microorganisms in vivo.

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